76-54-0 , 2,7-Dichlorofluorescein; 2',7'-Dichloro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; 2',7'-Dichloro-3,6-fluorandiol

76-54-0, 2,7-Dichlorofluorescein;
2',7'-Dichloro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; 2',7'-Dichloro-3,6-fluorandiol
Cas:76-54-0
C20H10Cl2O5 / 401.20
MFCD00005047

2,7-Dichlorofluorescein

2',7'-Dichloro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; 2',7'-Dichloro-3,6-fluorandiol

Fluorophore used in biological assays and for labeling cells and tissues.

2',7'-Dichlorofluorescein (DCF) is a fluorescent dye that is commonly used to measure reactive oxygen species (ROS), which are important in various biological processes. DCF is an analog of fluorescein and contains two chlorine atoms located at the 2' and 7' positions. The presence of these chlorine atoms makes DCF much less polar than fluorescein, making it more lipophilic and readily able to penetrate cell membranes. DCF has been widely used in various scientific applications, including biological research, environmental monitoring, and materials science.

CAS Number	76-54-0
Product Name	2',7'-Dichlorofluorescein
IUPAC Name	2',7'-dichloro-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
Molecular Formula	C20H10Cl2O5
Molecular Weight	401.2 g/mol
InChI	InChI=1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
InChI Key	VFNKZQNIXUFLBC-UHFFFAOYSA-N
SMILES	C1=CC=C2C(=C1)C(=O)OC23C4=CC(=C(C=C4OC5=CC(=C(C=C35)Cl)O)O)Cl
Synonyms	2’,7’-Dichlorofluorescein; 2’,7’-Dichlorofluoresceine; D and C Orange 25; D and C Orange No. 25; Dichlorofluorescein; Fluorescein 27; Fluorescein 548
Canonical SMILES	C1=CC=C2C(=C1)C(=O)OC23C4=CC(=C(C=C4OC5=CC(=C(C=C35)Cl)O)O)Cl